The pluripotency of MPSII line was confirmed by alkaline

2018-10-20

The pluripotency of MPSII-4.1 line was confirmed by alkaline phosphatase staining (ALP) and by immunocytochemistry staining (ICC) for endogenous NANOG and -CADHERIN (B) after silencing of the exogenous hOKSM construct. The spontaneous differentiation potential of the iPSC line towards the three ger

MaSC numbers must be tightly

2018-10-20

MaSC numbers must be tightly regulated through a balance of self-renewal, differentiation, and senescence to prevent tumorigenesis, while accommodating high levels of cell proliferation during pregnancy. Several secreted factors such as WNT and Hedgehog promote self-renewal, but little is known abou

With regard to prepubertal testicular tissue an important co

2018-10-20

With regard to prepubertal testicular tissue, an important concern for IVM is proper establishment of the blood testis barrier (BTB) during the peripubertal period. Several studies have demonstrated a correlation between dysfunction of the BTB and impaired spermatogenesis (Cavicchia et al., 1996), a

If nestin cells do generate new neurons and DA neurons

2018-10-20

If nestin+cells do generate new neurons and DA neurons within the microenvironment of the adult midbrain, knowledge about their ontogenesis will be crucial to identify signaling mechanisms regulating neurogenesis and DA neurogenesis here, which might help progress cell-replacement therapies for PD.

However it is likely that in addition to PU

2018-10-20

However, it is likely that, in addition to PU.1, other TFs participate in the initiation of the establishment of pre-existing enhancers and the activation of de novo enhancers. Thus, C/EBPα sites also were enriched for the RUNX motif, in line with the finding that during myelopoiesis Runx1 binds tra

Our gene chip analysis also revealed

2018-10-20

Our gene chip analysis also revealed a robust upregulation of metallothionein (MT) family members that are involved in metal metabolism and detoxification, as well as reactive oxygen species (ROS) scavenging. Overexpression of MT has been shown to protect mice from streptozotocin (STZ)-induced β cel

br Experimental Procedures See Supplemental Experimental Pro

2018-10-20

Experimental Procedures See Supplemental Experimental Procedures for details on mice, flow cytometry and cell sorting, cell-cycle and apoptosis analysis, myeloid progenitor and Lys-GFP in vitro differentiation assay, and gene expression. Author Contributions Acknowledgments Introduction

Hello world!

2018-07-29